l6S rDNA克隆文库方法分析两纯系鸡回肠及盲肠黏膜细菌的组成

doi:10.11843/j.issn.0366-6964.2014.08.003

Abstract

Abstract:

The experiment was conducted to study the composition and diversity of the mucosa-associated microbiota (MAM) in ileum and cecum between high quality chicken line A (pure line ♂，group A) and Huiyang Beard chicken (pure line ♂，group B) at 28 days old.Two chicken intestinal tracts were collected in group A and B，and mucosa-associated microbiota DNA were extracted at 28 days old，respectively.The random clone library of 16S rDNA V3 region of the mucosa-associated microbiota (MAM) in ileum and cecum between group A and B was established by analyzing 16S rDNA gene sequences.Analysis of the library in ileum showed that 6 operational taxonomic units (OTUs) (form 86 gene sequences or clones) were detected in the group A，as compared with 2 OTUs (form 97 clones) in the group B.Sequence being related to Enterococcus，Lactobacillus，Coprococcus and Clostridiaceae were found in the clone library of the MAM in ileum in group A.Clostridiaceae was only detected in group B.Analysis of the library in cecum shown that 44 OTUs (form 93 clones) were detected in the group A，as well as 42 OTUs(from 97 clones) in the group B.Unclassified Ruminococcaceae (18.28%),Coprococcus (13.98%)，Bacteroides (10.75%)，Blautia (10.75%) and Unclassified Lachnospiraceae (7.53%) were predominant in the group A，and Faecalibacterium (17.53%)，Coprococcus (11.34%)，Bacteroides (9.28%) and Unclassified Ruminococcaceae (8.25%) were abundant in the group B.The amount of other species found in cecum in group A were significant different from that in group B.In addition，sequence known being related to Colibacillus were not detected in group A and B.These results indicate that the composition of the MAM in ileum and cecum at day 28 days old are different between the high quality chicken line A and Huiyang Beard chicken，especially the proportion of butyric acid bacteria and Ruminococcus，and the host genetic factors may plays key role in this difference.

CLC Number:

S831.2

RONG Ting，ZHANG Jie，WANG Gang，LIU Zhi-chang，LI Shu-hong，LIN Hai-dan，CHEN Zhuang. Analysis of Composition of the Mucosa-associated Microbiota in Ileum and Cecum between Two Chicken Pure Lines by 16S rDNA Clone Library[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1218-1227.

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Analysis of Composition of the Mucosa-associated Microbiota in Ileum and Cecum between Two Chicken Pure Lines by 16S rDNA Clone Library

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